c6 36ht cells (ATCC)

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ATCC c6 36ht cells
C6 36ht Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1378 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c6+36+cells/Aedes+albopictus+clone+C6%2F36/pm42149960-510-0-2
Average 97 stars, based on 1378 article reviews

c6 36ht cells - by Bioz Stars, 2026-09

97/100 stars

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Isolation:

Article Title: Antibodies which specifically bind to dengue virus serotype 4 (DENV4) non-structural protein 1 (NS1) and methods of use thereof to detect a DENV4 infection
Article Snippet: Cloning DENV4 NS1 into pl-secSUMO* Vector. .. Total viral RNA was isolated from viral seeds of DENV4 (H421, a prototype strain) infected C6/36 cells (Aedes albopictus cell line, ATCC®) using the QIAAMP® Viral RNA Mini kit (QIAGEN®, Gaithersburg, MD, USA), and a complimentary DNA (cDNA) of DENV4 NS1 gene was reverse transcribed using SUPER SCRIPTTM III one step reverse transcriptase polymerase chain reaction (RT PCR) kit (INVITROGENTM, Carlsbad, CA, USA) and paired primers (forward: 5′- ATACGTCTCTAGGTGACACGGGTTGTGCGGTG-3′; SEQ ID NO: 17) and (reverse; 5′- GCGTCTAGATTAGGCCGATACCTGTGATTT-3; SEQ ID NO: 18), according to manufacturer's instructions. .. These primers were designed to introduce restriction sites for BmsBI and XbaI in order to clone the full length of DENV4 NS1 gene into a directional pl-secSUMO* cloning vector (LifeSensors, Malvern, PA).

Article Title: Identification of the First CHeRI Orbivirus 3-5 Strain Isolated from a Dead Farmed White-Tailed Deer ( Odocoileus virginianus) Whose Death Had Been Attributed to an Infection by Mule Deerpox Virus.
Article Snippet: .. Virus isolation was attempted in Vero E6 (Cercopithecus aethiops [African green monkey]) (Cat. no. CRL1586) and C6/36 cells (Aedes albopictus [Asian tiger mosquito]) (Cat. no. CRL1660) obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA), as described in [21]. .. The cells were propagated as monolayers in 25 cm2 vented tissue culture flasks (25 cm2 flask, Corning Inc., Corning, NY, USA) using Advanced Dulbecco’s Modified Eagle’s Medium (aDMEM, Invitrogen Corp. Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 2 mM L-alanyl-L-glutamine (GlutaMAXTM, Invitrogen Corp.), antibiotics (PSN; 50 μg/mL penicillin, 50 μg/mL streptomycin, 100 μg/mL neomycin [Invitrogen Corp.]), and 10% low-antibody, heat-inactivated, gamma-irradiated fetal bovine serum (FBS, Hyclone, GE, Healthcare Life Sciences, Pittsburgh, PA, USA).

Infection:

Reverse Transcription:

Polymerase Chain Reaction:

Reverse Transcription Polymerase Chain Reaction:

Modification:

Article Title: Defining the impact of Getah virus envelope protein glycosylation site mutations on viral replication, host adaptation, virulence, and immune evasion.
Article Snippet: BHK-21 cells (ATCC, CCL-10, RRID: CVCL_1914) and Vero cells (ATCC, CCL-81, RRID: CVCL_0059) were purchased from American Type Culture Collection (ATCC) and cultured using established protocols. .. C6/36 cells (ATCC, CRL-1660, RRID: CVCL_Z230) were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and L-glutamine at 28°C under 5% CO2. ..

Article Title: Defining the impact of Getah virus envelope protein glycosylation site mutations on viral replication, host adaptation, virulence, and immune evasion
Article Snippet: BHK-21 cells (ATCC, CCL-10, RRID: CVCL_1914) and Vero cells (ATCC, CCL-81, RRID: CVCL_0059) were purchased from American Type Culture Collection (ATCC) and cultured using established protocols. .. C6/36 cells (ATCC, CRL-1660, RRID: CVCL_Z230) were purchased from ATCC and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and L-glutamine at 28°C under 5% CO2. ..

Cell Culture:

Article Title: An ovary-enriched piRNA controls ovarian development via Gadd45a-JNK-mediated nurse-cell apoptosis in the arbovirus vector Aedes albopictus.
Article Snippet: The Asian tiger mosquito (Aedes albopictus) is a rapidly expanding invasive species and a global vector of arboviruses.. Its transmission depends on the female’s blood meal-driven reproductive cycle.. Although mosquito PIWI-interacting RNAs (piRNAs) research has predominantly focused on antiviral immunity, their roles in reproduction remain poorly understood.

Article Title: Apolipoprotein E drives inflammation resolution pathways essential for recovery from Japanese encephalitis
Article Snippet: .. Vero E6 (C1008, ECACC, Wiltshire, England; obtained via Sigma Aldrich, St. Louis, MO, USA) and C6/36 cells (ATCC# CRL-1660) were cultured in medium comprising RPMI 1640 (Gibco), supplemented with 10% foetal bovine serum (FBS), penicillin (100 IU/ml)/streptomycin (100 μg/ml) (Gibco/Life Technologies) and L-glutamine (2 mM) (Life Technologies). ..

other:

Article Title: Spectroscopic fingerprinting of extracellular vesicles from diverse cellular origins by ATR-FTIR for vibrational biomarkers of vector-host interactions.
Article Snippet: Primary HDF cells were obtained from CELLnTEC (CnT Dermal Fibroblasts, Juvenile, Single Donor); C6/36 cells (ATCC CRL-1660) were provided by the Institute for Virology, Charité, Berlin, Germany, and Huh7 cells were provided by the Department for General and Visceral Surgery, Ulm University, Ulm, Germany.

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